Comparative Genomic Hybridization (CGH) Microarray

CGH analysis of genomic DNA from human or mouse cells utilizing Agilent arrays and analyzed with CytoGenomics software. Assay performed by WiCell Cytogenetics and reviewed by an ABMG-certified director, with results available in 10-15 days.

What it detects:

  • Genomic gains and losses 
    • Copy number variants (CNVs)
    • Duplications/deletions
    • Unbalanced translocations
    • Aneuploidies
  • >20% mosaicism (for example: cultures where >1 of 5 cells are trisomy 12)

What it doesn’t detect:

  • Balanced translocations
  • Inversions
  • <20% culture mosaicism (for example: cultures where 1 of 5 cells is trisomy 12)
  • Chromosomal position of genomic gains
  • Loss of heterozygosity (LOH) / Absence of heterozygosity (AOH)

When to use:

  • As a baseline genomic screen
    • To detect submicroscopic (<5Mb) abnormalities
    • To identify amplified or deleted genes of interest
  • In conjunction with g-banded karyotyping
    • To define translocation breakpoints
  • For research of genomic copy number change
    • To identify structural variation within populations or disease cohorts
    • To develop a cell line copy number variant profile