WiCell and The WISC Bank

Human Pluripotent Stem Cell Culture Methods Course
Section 10-05 05/10/2010 to 05/12/2010 Fee: $2,000.00	* Required information

Does your lab have human embryonic stem cell lines?	yes no
From which institution did you order and obtain them?

What cell or human embryonic stem cell culture experience do you possess? *
	Regularly	Sometimes	Never
I understand the theory of cell culture, propagation and differentiation.
I currently culture mammalian cells.
I use mice or other animals as source of cellular or biological material.
I comply with safety and health regulations in my cell culture lab.
I use aseptic (sterile) technique and understand its principle.
I make cell culture media.
I make other cell culture reagents.
I characterize and observe cells by Microscopy and morphology
I freeze and thaw cell stocks.
I grow, split and passage cells.
I grow, split and passage human embryonic stem cells.
I derive mouse embryo fibroblasts.
I prepare mouse embryonic feeder layers.
I maintain and select differentiated human embryonic stem cells.
I conduct experiments using human embryonic stem cells.
I know how to use a Drummond Automatic Pipet-Aid, or equivalent.

Name 3-4 skills that you wish to acquire by taking this course. *
What do you want to be able to do after you complete the course and return to your lab? *
What is the focus, in general, of your lab's research? *

Human Pluripotent Stem Cell Culture Methods Course