• Culturing hES cells in an MEF feeder system
• Culturing hES and iPS cells in a feeder independent system
• Freezing and thawing of hES and iPS cells cultured in a feeder independent system
• Generation of Embryoid Bodies (EBs) from hES and iPS cells cultured in a feeder independent system
  

Expert Stem Cell Instruction

The teaching staff at WiCell provides technical expertise and qualified assistance throughout the course. This course offers a teacher:student ratio of 1:3. This low ratio is conducive to learning, and for that reason, we restrict the size of classes. Course instructors are highly experienced in culturing both hES and iPS cells on a daily basis, and are therefore qualified to address the many difficulties encountered when working with these cells. Upon completion of the course, our skilled instructors and technical support team are able to offer further assistance.

A course manual will be provided to each participant that includes additional support materials and protocols including:

• MEF Derivation and Freezing
• Culturing cells in Conditioned Medium (CM)
• Poly-Herma Coating of Flasks

Links to Pictures:

hES colony cultured in mTeSR medium

iPS colony cultured in mTeSR medium

EBs derived from hES cells cultured in mTeSR medium