Cell Suspension Culture
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Human Embryonic Stem (hES) cells are typically grown as adherent colonies attached to the bottom of a tissue culture dish (2 dimensional cell culture). While this method of culturing hES cells is satisfactory for small scale production and experimentation, it is extremely labor intensive and therefore is an inefficient method for obtaining the large number of hES cells that would be required for use in drug screening and clinical transplantation therapies. Several other mammalian cell types can be successfully grown as non-adherent cells suspended in culture medium. This 3-dimensional culture system allows cells to be grown at greater cell densities and in much greater volumes than traditional 2-dimensional adherent culture systems; therefore making suspension culture much more efficient. For this reason, WiCell Research Institute is currently investigating techniques to grow hES cells in a 3-dimensional suspension culture system.
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