In this section, we provide updated protocols and information to assist you with your human embryonic stem cell culture. These are the same protocols that are being followed in our production and distribution laboratory to successfully grow and expand our hES cell lines.
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Protocol I -- Derivation of Mouse Embryonic Fibroblasts
720 KB

Protocol II -- Culture and Propagation of Mouse Embryonic Fibroblasts
1.3 MB

Protocol III -- Thawing & Plating Human Embryonic Stem Cells
1.2 MB

Protocol IV -- Splitting Human Embryonic Stem Cells
2.4 MB

Protocol V -- Selection and Maintanence of Human Embryonic Stem Cells
656 KB

Please note: Because of the size of this file, we have removed graphics. They can be downloaded by selecting any or all of the next four links.

Protocol V - Differentiation Figs, A-B

Protocol V - Differentiation Figs, C-D

Protocol V - Figs. 4-5

Protocol V - Figure 6

Protocol VI - Freezing Human ES Cells
508 KB

Appendix - Gelatin Coating of Plates
43 MB

Notes on Freezing and Testing MEFs

Work Flow Diagram
This two-page diagram provides a sample time-frame for setting up hES cell culture.