This reporter cell line is a modified version of BG01, engineered to insert an enhanced GFP (EGFP) cassette into the Olig2 locus. R-Olig2 remained pluripotent and allowed for direct visualization of Olig2 expression by fluorescence microscopy and purification of Olig2+ precursors by fluorescence-activated cell sorting (FACS). This cell line can be used as a tool to study motoneuron and oligodendrocyte lineage development.
- Cell Line Alias R-Olig2
- Unit of Measure (UOM) Vial
- Cell Type Modified Human ES
- Sex Male
- Genetic Modification Keyword Reporter; Neural
- Disease None reported
- Pubmed Abstract Xue, Haipeng et al. "A Targeted Neuroglial Reporter Line Generated by Homologous Recombination in Human Embryonic Stem Cells." Stem Cells 27.8 (2009): 1836-1846.
- Provider Life Technologies
- Collections Uncategorized
Lot Number | Culture Platform | Lot Description | Banked By | Passage Number | Protocol | Product Info URL |
DB0004 | StemPro/Geltrex | Olig2-GFP reporter BG01 line by Homologous recombination. This lot was produced using the provider’s protocol. | Provider | 19(3) | Life Technologies LT1e-OLIG2GFP Protocol |