Enabling investigators to establish stem cell lines in their laboratories is the primary goal of the WiCell Stem Cell. To this aim, WiCell provides protocols for every available stem cell line. The protocols are also shipped with every vial of material. Many cell lines are available in multiple culture platforms (i.e., combinations of medium and matrix). Culture platforms and associated protocols for each cell line are accessible when viewing cell line specific information.
WiCell recommends that investigators review protocols and contact our Technical Services Team with any questions prior to thawing the material.
Feeder Independent Protocols
WiCell Feeder Independent Pluripotent Stem Cell Protocol
Supplement: Culture with CultureCEPT
Supplement: Culture with Doxycycline
Supplement: Culture with G418
Supplement: Culture with Imatinib
Supplement: Culture with Zeocin
Supplement: Culture with Zeocin and Hygromycin
WiCell Feeder Independent Pluripotent Stem Cell Protocol with Supplement Culturing with G418
Feeder Free Protocols (Conditioned Media)
Feeder Dependent Protocols (MEF Based)
WiCell Feeder Based (MEF) Pluripotent Stem Cell Protocol
WiCell Feeder Based (MEF) Protocol 01 for Culture of MJFF iSCORE Lines
Supplement: Culture of Elf1 Cells
Other Protocols
WiCell Embryonal Carcinoma Cell Line GCT27C4 Protocol
WiCell Embryonal Carcinoma Cell Line GCT27DC1 Protocol
WiCell Neural Stem Cell Protocol