Powered by BiozThis cell line is a modified version of WISC011i-inGFPpuro, engineered using CRISPR/Cas9 with dual gRNA to edit the CCR5 gene. This cell line may be used to study of the role of CCR5 in HIV infection in vitro, and generation of HIV-resistant cells for potential therapeutic applications.
- Cell Line Alias GFP-CCR5mut-hiPSC-Comb2-SC1
- Unit of Measure (UOM) vial
- Cell Type Modified Human iPS
- Sex Male
- Reprogramming Method CRISPR/CAS9 technology using Addgene Plasmid ID 43861: mammalian codon optimized Cas9 nuclease (JDS246) Addgene Plasmid ID 43860: guide RNA (gRNA) expression vector
- Genetic Alteration Mutation Information Biallelic 551bp deletion in CCR5 gene.
- Genetic Modification Keyword Isogenic; HIV
- Disease None reported
- Ethnicity African American > Nigerian
- Genetic Alteration Mutation CCR5
- Pubmed Abstract Kang, Hyunjun, et al "CCR5 Disruption in Induced Pluripotent Stem Cells Using CRISPR/Cas9 Provides Selective Resistance of Immune Cells to CCR5-tropic HIV-1 Virus." Molecular Therapy Nucleic Acids,vol. 4 (2015), doi:10.1038/mtna.2015.42.
- Provider University of Wisconsin - Dr. Igor Slukvin
- Collections Uncategorized
| Lot Number | Culture Platform | Lot Description | Banked By | Passage Number | Protocol | Product Info URL |
| WB66706 | TeSR-E8/Matrigel | WiCell | 53 | WiCell Feeder Independent Pluripotent Stem Cell Protocol |