About Topol Lab’s Next Gen Cell Lines

This collection, from Dr. Eric Topol (Scripps), was generated to elucidate cardiovascular phenotypes employing genome editing of iPS cells.

The iPS cell lines in this collection were banked and characterized in the laboratories of Dr. Kristin Baldwin, Scripps Research Institute and Dr. Sheng Ding, Gladstone Institutes.

This collection contains 97 cell lines and includes genotyping of three sites within the 9p21 region for each donor. Disease states for the donors include coronary artery disease and myocardial infarction as well as individuals with no reported diagnosis. The age of donors range from 23-100 and ethnicities include Caucasian, European, and Latino.

About the Next Generation Genetic Association Studies (Next Gen) Program

These cell lines were created as Next Generation Genetic Association Studies (Next Gen) Program, which was a five-year, $80 million program to investigate functional genetic variation in humans by assessing cellular profiles that are surrogates for disease phenotypes. To achieve this, researchers from multiple institutions across the U.S. were awarded grants to derive iPS cell lines from more than 1,500 individuals representing various conditions as well as healthy controls for use in functional genomic (‘disease in a dish’) research. This extensive panel includes a diverse set of age, gender and ethnic backgrounds, and therefore will be an invaluable tool for evaluations across demographics. Further enhancing the utility of these cell lines are data sets such as phenotyping, GWAS, genome sequencing, gene expression and -omics analyses (e.g., lipidomic, proteomic, methylomic) that will be made available with the cell lines.