Powered by BiozThis cell line is a modified version of IMR90-TSC2Het. It also serves as an engineered counterpart to WC-52-TSC2Null. The TSC2 mutation was introduced to IMR90-TSC2Het in the unaffected allele to create null TSC2-/- iPSCs via CRISPR-Cas9. Isogenic cell lines iPS(IMR90-4) and IMR90-TSC2Het are available from WiCell.
- Cell Line Alias IMR90-TSC2-/-
- Unit of Measure (UOM) vial
- Cell Type Modified Human iPS
- Sex Female
- Reprogramming Method Non-Integrating (Sendai: Oct4, Klf4, Sox2, cMyc)
- Genetic Alteration Mutation Information C972G/C972G
- Genetic Modification Keyword Isogenic - Tuberous Sclerosis Complex
- Disease Tuberous Sclerosis Complex
- Genetic Alteration Mutation TSC2 (homozygous)
- Pubmed Abstract Catlett, Timothy S et al. ``RHOA signaling defects result in impaired axon guidance in iPSC-derived neurons from patients with tuberous sclerosis complex.`` Nature communications 12.1 (2021), doi:10.1038/s41467-021-22770-4.
- hPSCReg ID WISCi004-B-8
- Provider University of Wisconsin - Dr. Timothy Gomez
- Collections Tuberous Sclerosis Complex Collection
| Lot Number | Culture Platform | Lot Description | Banked By | Passage Number | Protocol | Product Information & Testing |
| WB67713 | mTeSR Plus/Matrigel | WiCell | 43 | WiCell Feeder Independent Pluripotent Stem Cell Protocol |