The provider notes the following: The donor for this cell line has been genetically confirmed to be heterozygous, and to express dysferlin at a lower level than the donor for the WT line. However, for reasons that are unclear, the Romeo antibody (Epitomics #5140-1) which targets the N-terminal region of dysferlin, does not show reactivity with muscle cells derived from this cell line. An alternate dysferlin antibody (Leica NCL-Hamlet) targeting the C-terminal region of dysferlin does show reactivity with muscle cells derived from this cell line. Bear this in mind when designing experiments using this cell line.

Additional clones from this iPS cell line (JFHZ2, JFHZ3, JFHZ4 and JFHZ6) are available from WiCell.

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Lot Number Culture Platform Lot Description Banked By Passage Number Protocol Product Info URL
WB66587 TeSR-E8/Matrigel WiCell 9 WiCell Feeder Independent Pluripotent Stem Cell Protocol PDF

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