Powered by BiozThis cell line is a modified version of WC-52-TSC2Het iPSCs, engineered by introducing a TSC2 mutation in the unaffected allele of WC-52-TSC2Het via CRISPR-Cas9. Isogenic cell lines WC-52-TSC2Corr and WC-52-TSC2Het, as well as engineered counterparts IMR90-TSC2Het and IMR90-TSC2Null are available from WiCell.
- Cell Line Alias TSC2-/-
- Unit of Measure (UOM) vial
- Cell Type Modified Human iPS
- Sex Male
- Age at Collection 18 years
- Reprogramming Method Non-Integrating (Sendai: Oct4, Klf4, Sox2, cMyc)
- Tissue Origin Skin Fibroblast
- Genetic Alteration Mutation Information C972G/C972G
- Genetic Modification Keyword Isogenic - Tuberous Sclerosis Complex
- Disease Tuberous Sclerosis Complex
- Genetic Alteration Mutation TSC2 (homozygous)
- Pubmed Abstract Catlett, Timothy S et al. ``RHOA signaling defects result in impaired axon guidance in iPSC-derived neurons from patients with tuberous sclerosis complex.`` Nature communications 12.1 (2021), doi:10.1038/s41467-021-22770-4.
- hPSCReg ID WISCi019-A-2
- Provider University of Wisconsin - Dr. Timothy Gomez
- Collections Tuberous Sclerosis Complex Collection
| Lot Number | Culture Platform | Lot Description | Banked By | Passage Number | Protocol | Product Information & Testing |
| WB67719 | mTeSR Plus/Matrigel | WiCell | 20 | WiCell Feeder Independent Pluripotent Stem Cell Protocol |