This reporter cell line is a modified version of WA09 (H9), engineered to use chromatin insulator elements to flank single-copy transgenes and integrate the combined expression constructs via PhiC31/R4 integrase-mediated recombination technology to the chromosome 13 locus precisely. Two copies of cHS4 double-insulator sequences were placed adjacent to both 5′ and 3′ of the promoter reporter constructs. The green fluorescent protein (GFP) gene is driven CMV early enhancer/chicken beta-actin (CAG) promoter. This results in constitutive GFP expression at the chromosome 13 locus that is maintained in prolonged culture. This cell line is a valuable tool for lineage development study, gain- and loss-of-function experiments, and human disease modeling using hESCs.