LT2e-H9CAGGFP
This reporter cell line is a modified version of WA09 (H9), engineered to use chromatin insulator elements to flank single-copy transgenes and integrate the combined expression constructs via PhiC31/R4 integrase-mediated recombination technology to the chromosome 13 locus precisely. Two copies of cHS4 double-insulator sequences were placed adjacent to both 5' and 3' of the promoter reporter constructs. The green fluorescent protein (GFP) gene is driven CMV early enhancer/chicken beta-actin (CAG) promoter. This results in constitutive GFP expression at the chromosome 13 locus that is maintained in prolonged culture. This cell line is a valuable tool for lineage development study, gain- and loss-of-function experiments, and human disease modeling using hESCs.
Cell Type
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Modified Human ES |
Disease
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None reported |
Genetic Modification Keyword
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Reporter; Constitutive |
Sex
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Female |
Genetically Related Cell Lines
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Provider
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Life Technologies
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dbGaP Data
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hPSCReg ID
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NIH Approval
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Pub Med Abstract
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Related Products
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Collection(s)
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NIH Approved
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