This reporter cell line is a modified version of WA09 (H9), engineered to use chromatin insulator elements to flank single-copy transgenes and integrate the combined expression constructs via PhiC31/R4 integrase-mediated recombination technology to the chromosome 13 locus precisely. Two copies of cHS4 double-insulator sequences were placed adjacent to both 5' and 3' of the promoter reporter constructs. The green fluorescent protein (GFP) gene is driven CMV early enhancer/chicken beta-actin (CAG) promoter. This results in constitutive GFP expression at the chromosome 13 locus that is maintained in prolonged culture. This cell line is a valuable tool for lineage development study, gain- and loss-of-function experiments, and human disease modeling using hESCs.
Cell Type Modified Human ES
Disease None reported
Genetic Modification Keyword Reporter; Constitutive
Sex Female
Genetically Related Cell Lines  
Provider Life Technologies
dbGaP Data  
hPSCReg ID  
NIH Approval  
Pub Med Abstract  
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Collection(s) NIH Approved
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Current Lot Information

Lot Number Culture Platform Lot Description Passage Number Banked By Protocol Product Information & Testing
WB0207 mTeSR1/Matrigel   75 WiCell WiCell Feeder Independent Pluripotent Stem Cell Protocol with Supplement Culturing with Hygromycin and Zeocin PDF

Historical Lot Information

Lot Number Culture Platform Lot Description Banked By Protocol Product Information & Testing
DB0001 Stem Cell Culture Medium (KOSR/MEF) Constitutive GFP reporter H9 cell line by homologous recombination. Provider Life Technologies LT2e-H9CAGGFP Protocol PDF