WISC014i-SC1
This cell line is a modified version of WISC011i-inGFPpuro, engineered using CRISPR/Cas9 with dual gRNA to edit the CCR5 gene. This cell line may be used to study of the role of CCR5 in HIV infection in vitro, and generation of HIV-resistant cells for potential therapeutic applications.
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Cell Line Alias
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GFP-CCR5mut-hiPSC-Comb2-SC1 |
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Cell Type
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Modified Human iPS |
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Disease
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None reported |
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Genetic Alteration/Mutation
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CCR5 |
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Genetic Alteration/Mutation Information
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Biallelic 551bp deletion in CCR5 gene. |
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Genetic Modification Keyword
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Isogenic; HIV |
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Sex
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Male |
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Ethnicity
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African American > Nigerian |
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Genetically Related Cell Lines
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Reprogramming Method
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CRISPR/CAS9 technology using Addgene Plasmid ID 43861: mammalian codon optimized Cas9 nuclease (JDS246) Addgene Plasmid ID 43860: guide RNA (gRNA) expression vector |
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Provider
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University of Wisconsin - Dr. Igor Slukvin
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dbGaP Data
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hPSCReg ID
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NIH Approval
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Pub Med Abstract
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Related Products
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